Thermo Fisher Scientific Phospho-CRYAB (Ser45) Polyclonal Antibody
Phospho-CRYAB (Ser45) 항체로 인산화된 alpha-B crystallin을 특이적으로 검출. Western blot, IHC, IF 등 다양한 응용 가능. Rabbit polyclonal IgG 형태로 제공되며, -20°C에서 보관. 항원 친화 크로마토그래피로 정제된 고품질 연구용 항체.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 8 µg/mL | View 1 publication |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to residues L(44) S(p) P F Y L R P P S F(54) C of human alpha-B Crystallin |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 30 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2084437 |
Product Specific Information
PA1-011 detects phospho-alpha-B crystallin (Ser45) from bovine, human, mouse, and rat samples.
This antibody does not detect the unphosphorylated form of the protein.
It has been successfully used in Western blot, immunohistochemistry, and immunofluorescence procedures.
- By Western blot, detects an ~20 kDa protein representing recombinant bovine phospho-alpha-B crystallin (Ser45).
- Immunofluorescence staining in U373 MG cells shows cytoplasmic localization.
- Immunogen: synthetic phosphopeptide corresponding to residues L(44) S(p) P F Y L R P P S F(54) C of human alpha-B Crystallin.
- Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.
Target Information
Lens proteins consist almost entirely of crystallins (about 95%), which also appear in vertebrate skeletal muscle tissue.
Crystallins maintain the lens refractive index and include three major classes: alpha, beta, and gamma.
- Alpha-crystallin: composed of alpha-A and alpha-B gene products; ~40% of total lens protein.
- Beta-crystallins: at least 5 variants.
- Gamma-crystallins: monomeric, with at least 5 identified in bovine and rat lens.
Alpha-crystallin acts as a molecular chaperone, preventing protein aggregation that may cause cataracts.
Phosphorylated alpha-crystallin responds to cellular stress and contributes to protein maintenance.
Alpha-B crystallin accumulation is associated with Alexander’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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