Thermo Fisher Scientific B4GALT4 Monoclonal Antibody (OTI8B6), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The B4GALT4 gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes that encode type II membrane-bound glycoproteins. These enzymes specifically use UDP-galactose as a donor substrate and transfer galactose in a β1,4 linkage to acceptor sugars such as GlcNAc, Glc, and Xyl.
Each beta4GalT has distinct roles in the biosynthesis of glycoconjugates and saccharide structures.
As type II membrane proteins, they possess an N-terminal hydrophobic signal sequence directing localization to the Golgi apparatus, functioning as a transmembrane anchor.
By sequence similarity, beta4GalTs form four groups:

• beta4GalT1 & beta4GalT2
• beta4GalT3 & beta4GalT4
• beta4GalT5 & beta4GalT6
• beta4GalT7

The enzyme encoded by B4GALT4 is mainly involved in glycolipid biosynthesis. Two alternatively spliced transcript variants have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.