Thermo Fisher Scientific IL-17F Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MRKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS MSRNIESRST SPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN SVPIQQETLV VRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-17F. Anti-Human IL-17F-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hIL-17F by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-17F (500-P90) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hIL-17F.

Western Blot:
To detect hIL-17F by Western Blot analysis, use at 0.1–0.2 µg/mL.
Used with compatible secondary reagents, the detection limit for Recombinant hIL-17F is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P90BT-1MG will be provided as 2 × 500 µg.

Target Information

IL-17F (Interleukin 17F, CTLA-8) is a cytokine belonging to the IL-17 family, produced by inflammatory cells such as activated T cells, mast cells, and basophils.
IL-17F is involved in allergic airway inflammation and induces cytokines, chemokines, and adhesion molecules in bronchial epithelial cells, vein endothelial cells, fibroblasts, and eosinophils.
It may be secreted as a homodimer or heterodimer with IL-17A, acting via IL-17R to recruit monocytes and neutrophils by increasing chemokine production.
IL-17F also stimulates TNF-α, IL-1β, IL-6, and IL-8 expression, and is implicated in chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and multiple sclerosis.
TGF-β and IL-23 are required for induction and maintenance of Th17 cells, which induce other pro-inflammatory cytokines.
IL-17F inhibits angiogenesis and induces endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.