Thermo Fisher Scientific CXCL1 Polyclonal Antibody, PeproTech
인간 CXCL1(GRO-alpha/MGSA)에 특이적인 Rabbit Polyclonal 항체로, Western blot 및 ELISA 등 다양한 분석에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 2 publications |
| In vitro Assay (IV) | – | View 1 publication |
| Immunostaining (IS) | – | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human GRO-alpha/MGSA (CXCL1) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930857 |
Product Specific Information
AA Sequence of recombinant protein:
ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-alpha/MGSA (CXCL1). Anti-Human GRO-alpha/MGSA (CXCL1)-specific antibody was purified by affinity chromatography employing an immobilized Human GRO-alpha/MGSA (CXCL1) matrix.
Sandwich ELISA:
To detect Human GRO-alpha/MGSA (CXCL1) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. When used with PeproTech Biotinylated Anti-Human GRO-alpha/MGSA (CXCL1) (500-P92Bt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Human GRO-alpha/MGSA (CXCL1).
Western Blot:
For Western Blot analysis, use at 0.1–0.2 µg/mL. With compatible secondary reagents, detection limit for Recombinant Human GRO-alpha/MGSA is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
CXCL1, CXCL2, and CXCL3—also known as GRO alpha, GRO beta (MIP-2 alpha), and GRO gamma (MIP-2 beta)—are members of the CXC chemokine subfamily. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, processed forms GRO-alpha(4–73), GRO-alpha(5–73), and GRO-alpha(6–73) exhibit approximately 30-fold higher chemotactic activity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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