Thermo Fisher Scientific CXCL5 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human ENA-78 (CXCL5). Anti-Human ENA-78 (CXCL5)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hENA-78 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human ENA-78 (500-P91) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hENA-78.

Western Blot:
To detect hENA-78 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hENA-78 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P91BT-1MG is provided as 2 × 500 µg.

Target Information

The protein encoded by this gene is an inflammatory chemokine belonging to the CXC chemokine family. It is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine acts as a potent chemotaxin involved in neutrophil activation.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.