Thermo Fisher Scientific CXCL5 Polyclonal Antibody, PeproTech

Applications

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human ENA-78 (CXCL5). Anti-Human ENA-78 (CXCL5)-specific antibody was purified by affinity chromatography employing an immobilized Human ENA-78 (CXCL5) matrix.

Sandwich ELISA:
To detect Human ENA-78 (CXCL5) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human ENA-78 (500-P91Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human ENA-78 (CXCL5).

Western Blot:
To detect hENA-78 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hENA-78 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

The protein encoded by this gene is an inflammatory chemokine belonging to the CXC chemokine family. It is produced concomitantly with interleukin-8 (IL8) in response to stimulation with interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). CXCL5 acts as a potent chemotaxin involved in neutrophil activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.