Thermo Fisher Scientific CXCL5 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.5-2.0 µg/mL

-

Immunostaining (IS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human ENA-78 (CXCL5) (5-78 a.a.) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CXCL5,uniProtId:P42830-1,ncbiNodeId:9606,antigenRange:41-114,antigenLength:114,antigenImageFileName:500-P91-100UG_CXCL5_P42830-1_Rabbit.svg,antigenImageFileNamePDP:500-P91-100UG_CXCL5_P42830-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930851

Product Specific Information

AA Sequence of recombinant protein: AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human ENA-78 (CXCL5). Anti-Human ENA-78 (CXCL5)-specific antibody was purified by affinity chromatography employing an immobilized Human ENA-78 (CXCL5) matrix.

Sandwich ELISA: To detect Human ENA-78 (CXCL5) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human ENA-78 (500-P91Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human ENA-78 (CXCL5).

Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

The protein encoded by this gene is an inflammatory chemokine that belongs to the CXC chemokine family. This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.