Thermo Fisher Scientific CXCL5 Polyclonal Antibody, PeproTech
CXCL5 단백질을 표적으로 하는 Rabbit Polyclonal Antibody로, Western blot, ELISA, Immunostaining 등에 사용 가능. 고순도 재조합 Human ENA-78(CXCL5) 항원으로 면역하여 제작되었으며, 항원 친화 크로마토그래피로 정제됨. 연구용으로만 사용.
- 카탈로그번호
- 500-P91-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CXCL5 Polyclonal Antibody, PeproTech
Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | - |
| Immunostaining (IS) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human ENA-78 (CXCL5) (5–78 a.a.) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930851 |
Product Specific Information
AA Sequence of recombinant protein:
AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human ENA-78 (CXCL5). Anti-Human ENA-78 (CXCL5)-specific antibody was purified by affinity chromatography employing an immobilized Human ENA-78 (CXCL5) matrix.
Sandwich ELISA:
To detect Human ENA-78 (CXCL5) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human ENA-78 (500-P91Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human ENA-78 (CXCL5).
Western Blot:
To detect hENA-78 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hENA-78 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The protein encoded by this gene is an inflammatory chemokine belonging to the CXC chemokine family. It is produced concomitantly with interleukin-8 (IL8) in response to stimulation with interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). CXCL5 acts as a potent chemotaxin involved in neutrophil activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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