Thermo Fisher Scientific CXCL5 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human ENA-78 (CXCL5). The anti-Human ENA-78 (CXCL5)-specific antibody was purified by affinity chromatography and subsequently biotinylated.

Sandwich ELISA:
To detect hENA-78 by sandwich ELISA (100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody. When used with PeproTech Polyclonal Anti-Human ENA-78 (500-P91) as a capture antibody, it allows detection of at least 0.2–0.4 ng/well of recombinant hENA-78.

Western Blot:
For Western blot analysis, use at 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for recombinant hENA-78 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Package Information:
500-P91BT-1MG will be provided as 2 × 500 µg.

Target Information

The CXCL5 gene encodes an inflammatory chemokine belonging to the CXC chemokine family. It is co-produced with interleukin-8 (IL8) in response to stimulation by interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). CXCL5 functions as a potent chemotaxin involved in neutrophil activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.