Thermo Fisher Scientific CXCL5 Polyclonal Antibody, Biotin, PeproTech
CXCL5(ENA-78)을 인식하는 Biotin 표지 토끼 다클론 항체로, ELISA와 Western blot에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 인간 시료 반응성을 가집니다. 냉동(-20°C) 보관, PBS buffer 기반, 방부제 무첨가.
- 카탈로그번호
- 500-P91BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CXCL5 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human ENA-78 (CXCL5) (5–78 a.a.) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930855 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human ENA-78 (CXCL5). The anti-Human ENA-78 (CXCL5)-specific antibody was purified by affinity chromatography and subsequently biotinylated.
Sandwich ELISA:
To detect hENA-78 by sandwich ELISA (100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody. When used with PeproTech Polyclonal Anti-Human ENA-78 (500-P91) as a capture antibody, it allows detection of at least 0.2–0.4 ng/well of recombinant hENA-78.
Western Blot:
For Western blot analysis, use at 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for recombinant hENA-78 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Package Information:
500-P91BT-1MG will be provided as 2 × 500 µg.
Target Information
The CXCL5 gene encodes an inflammatory chemokine belonging to the CXC chemokine family. It is co-produced with interleukin-8 (IL8) in response to stimulation by interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). CXCL5 functions as a potent chemotaxin involved in neutrophil activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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