Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), Super Bright 702, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), Super Bright 702, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue 610-70S, Blue 660-120S, Red 710, Yellow 610, Yellow 660
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation available

Product Specific Information

The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8 alpha/beta heterodimer or CD8 alpha/alpha homodimer. CD8a is found on thymocytes, mature T cells, and NK cells. It binds to MHC class I and associates with p56lck, playing a role in T-cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated for flow cytometry using normal human peripheral blood cells (5 µL or 0.06 µg/test)
• Super Bright 702: Tandem dye excited at 405 nm, emits at 702 nm (recommended 710/50 bandpass filter)
• Staining Panels: Use Super Bright Staining Buffer (SB-4400) to minimize polymer interactions
• Light Sensitivity: Protect from light due to photo-induced oxidation
• Fixation: Samples can be stored up to 3 days at 4°C in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) with minimal impact on brightness
• Laser: Violet (405 nm)

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein involved in signal transduction and T cell differentiation. CD8 is expressed on T cells as a CD8α/CD8β heterodimer, functioning as a co-receptor for MHC class I molecules. It also exists as CD8α homodimers on subsets of T cells, NK cells, monocytes, and dendritic cells. Interaction with MHC-I/peptide complexes recruits Lck kinase, leading to CTL activation and pathogen/tumor clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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