Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Super Bright 702, eBioscience
마우스 CD8a를 인식하는 53-6.7 클론의 Rat IgG2a 단일클론 항체로, Super Bright 702 형광체 결합. Flow cytometry에 최적화되어 있으며 0.25 µg/test 권장. Violet laser(405 nm)로 여기되어 702 nm에서 방출되며, T 세포 활성화 및 분화 연구에 적합.
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Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Super Bright 702, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 11 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 88 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 702, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-6.7 |
| Conjugate | Super Bright™ 702 |
| Excitation / Emission Max | 413 / 702 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2662351 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV, Violet, Yellow series
- eFluor 450, 506, 615, 660
- FITC
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 780
- NovaFluor Blue, Red, Violet, Yellow series
- Request custom conjugation
Product Specific Information
The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as either CD8αβ heterodimer or CD8αα homodimer. CD8 binds MHC class I and, through association with p56lck, plays a role in T cell development and activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometric analysis of mouse splenocytes (≤0.25 µg/test)
- Excitation/Emission: 405 nm / 702 nm (Violet laser, use 710/50 bandpass filter)
- Light Sensitivity: Protect from light
- Fixation: Samples can be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days at 4°C with minimal impact on brightness
- Recommended Buffer: Super Bright Staining Buffer (Product # SB-4400)
Super Bright 702 is a tandem dye excited by violet laser (405 nm) and emits at 702 nm. Ensure instrument compatibility.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein involved in signal transduction and T cell differentiation. CD8 functions as a co-receptor for MHC class I molecules and is expressed on T cells, NK cells, monocytes, and dendritic cells. Activation of CD8+ T cells leads to cytotoxic T lymphocyte (CTL) formation, essential for pathogen and tumor cell clearance. Differentiation is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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