Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Super Bright 702, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Super Bright 702, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV, Violet, Yellow series
• eFluor 450, 506, 615, 660
• FITC
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 780
• NovaFluor Blue, Red, Violet, Yellow series
• Request custom conjugation

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as either CD8αβ heterodimer or CD8αα homodimer. CD8 binds MHC class I and, through association with p56lck, plays a role in T cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse splenocytes (≤0.25 µg/test)
• Excitation/Emission: 405 nm / 702 nm (Violet laser, use 710/50 bandpass filter)
• Light Sensitivity: Protect from light
• Fixation: Samples can be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days at 4°C with minimal impact on brightness
• Recommended Buffer: Super Bright Staining Buffer (Product # SB-4400)

Super Bright 702 is a tandem dye excited by violet laser (405 nm) and emits at 702 nm. Ensure instrument compatibility.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein involved in signal transduction and T cell differentiation. CD8 functions as a co-receptor for MHC class I molecules and is expressed on T cells, NK cells, monocytes, and dendritic cells. Activation of CD8+ T cells leads to cytotoxic T lymphocyte (CTL) formation, essential for pathogen and tumor cell clearance. Differentiation is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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