Thermo Fisher Scientific VIM Monoclonal Antibody (OTI1A9), TrueMAB
Vimentin 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC/IF 등 다양한 응용에 적합합니다. Human 시료에 반응하며, Lyophilized 형태로 제공됩니다. 고순도 정제 및 안정적인 저장 조건을 갖춘 연구용 항체입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:150 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A9 |
| Immunogen | Full length human recombinant protein of human VIM produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Vimentin is a developmentally regulated intermediate filament protein found in cells of mesenchymal origin. It plays a role in intracellular transport between the nucleus and plasma membrane and is expressed during early cellular development along with desmin. Vimentin is involved in steroid synthesis regulation by mediating cholesterol transport within lipid droplets and mitochondria. Phosphorylation by protein kinase induces cytoskeletal rearrangements facilitating steroidogenic processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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