Thermo Fisher Scientific LDHA Polyclonal Antibody
LDHA 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 친화 크로마토그래피 정제. -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein corresponding to amino acids 1–332 of human LDHA (NP_005557.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.63 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804680 |
Product Specific Information
Immunogen Sequence:
MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF
Positive Samples: MCF7, 293T, NIH/3T3, K-562, Jurkat, Mouse skeletal muscle
Cellular Location: Cytoplasm
Target Information
Lactate dehydrogenase (LDH) is an enzyme in the glycolytic pathway that catalyzes the conversion of pyruvate to lactate with concomitant interconversion of NADH and NAD⁺. In mammals, LDH is encoded by three genes: LDHA (muscle form), LDHB (heart form), and LDHC (testis form).
The three human LDH isoforms share 84–89% sequence similarity and 69–75% amino acid identity.
There are five isoenzymes based on M and H chain composition in the tetrameric structure.
LDHA expression is hypoxia-inducible and regulated by HIF1α transcriptional activity. LDHB is often upregulated in cancers.
Mutations in LDHA can cause Glycogen Storage Disease 11 (GSD11).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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