Thermo Fisher Scientific Leptin Polyclonal Antibody, PeproTech
인간 렙틴을 검출하기 위한 고순도 폴리클로날 항체로, Western blot과 ELISA에 최적화되어 있습니다. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관 가능합니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- 500-P86-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Leptin Polyclonal Antibody, PeproTech
Thermo Fisher Scientific Leptin Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 3 publications |
| Radioimmune Assays (RIA) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Bovine, Human, Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human Leptin |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930815 |
Product Specific Information
AA Sequence of Recombinant Protein:
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Leptin. Anti-Human Leptin-specific antibody was purified by affinity chromatography employing an immobilized Human Leptin matrix.
Sandwich ELISA:
To detect Human Leptin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Leptin (500-P86Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human Leptin.
Western Blot:
To detect Human Leptin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human Leptin is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
Leptin is a 167 amino acid protein hormone with key roles in regulating body weight, metabolism, and reproductive function. It has a molecular weight of approximately 16 kDa and is encoded by the obese (ob) gene. Leptin is mainly secreted by adipocytes, with smaller amounts produced by stomach epithelium and placenta.
Leptin receptors are highly expressed in hypothalamic regions that regulate feeding behavior, hunger, body temperature, and energy expenditure. It is involved in hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, pubertal development, and fetal growth.
Studies have linked leptin to obesity, anorexia nervosa, insulin resistance, and hypertension. It also regulates fatty acid oxidation enzymes and thermogenesis. Defects in leptin production can cause severe hereditary obesity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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