Thermo Fisher Scientific Apolipoprotein B Monoclonal Antibody (F2C9)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• MIA1609 targets Apolipoprotein B in ELISA, IP, and RIA applications and reacts with Human samples.
• Immunogen: purified human serum LDL.
• Detects Apolipoprotein B (~502 kDa predicted molecular weight).
• Formerly sold as a Seradyn product.

Target Information

Apolipoprotein B (apo B) is the major protein of low-density lipoproteins (LDL) in human plasma, with a molecular mass of approximately 260–500 kDa. It binds to specific receptors on cell membranes and mediates the removal of LDL and very low-density lipoprotein (VLDL) cholesterol from circulation.

LDL serves as the carrier protein for cholesterol in the blood. In atherosclerotic lesions, oxidatively modified LDL is found and recognized by macrophages via scavenger receptors A and CD36. Oxidized LDL may serve as a marker of atherosclerosis.

Apolipoprotein B is synthesized mainly in the liver and is a major component of VLDL, intermediate density lipoproteins, LDL, and lipoprotein(a). It acts as a ligand for the LDL receptor, and elevated levels are associated with premature atherosclerosis. Normal plasma levels are approximately 800 mg/L.

Two main isoforms exist: apoB-48 (intestinal) and apoB-100 (hepatic), both encoded by a single gene. RNA editing of the apoB-100 transcript at residue 2180 results in apoB-48. Mutations in the Apolipoprotein B gene can cause disorders such as hypobetalipoproteinemia and hypercholesterolemia due to ligand-defective Apolipoprotein B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.