Thermo Fisher Scientific CD8 Monoclonal Antibody (38.65), FITC
Bovine, Goat, Sheep 시료에 반응하는 FITC 결합 CD8 단클론 항체. Flow Cytometry용으로 최적화되었으며, 38.65 클론 기반. 단기 4°C, 장기 -20°C 보관 권장. 세포 표면 CD8 항원 검출에 적합.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: Neat–1:10
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Goat, Sheep |
| Published Species | Bovine |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 38.65 |
| Immunogen | Ovine efferent lymphocytes |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; -20°C long term (avoid freeze/thaw). Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_929415 |
Additional Formats
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Mouse anti-Sheep CD8 antibody, clone 38.65 recognizes the ovine CD8 cell surface antigen, expressed by the cytotoxic/suppressor subset of T lymphocytes.
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family.
It plays an integral role in signal transduction and T cell differentiation and activation.
CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer (CD8α/CD8β) acting as a co-receptor for MHC class I molecules.
It is also found on subsets of T cells, NK cells, monocytes, and dendritic cells as CD8α homodimers.
Upon MHC-I/peptide complex binding, CD8 recruits Lck kinase, leading to lymphokine production and activation of cytotoxic T lymphocytes (CTLs).
Activated CTLs are essential for pathogen and tumor cell clearance. Differentiation of naïve CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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