Thermo Fisher Scientific GM-CSF Monoclonal Antibody (MP1-31G6), Biotin, eBioscience

Applications and Tested Dilution

• Application: ELISA
• Tested Dilution: 0.5–2 µg/mL
• Publications: View 1 publication

Product Specifications

Product Specific Information

Description:
The MP1-31G6 antibody reacts with mouse granulocyte/macrophage-colony stimulating factor (GM-CSF), a 14 kDa cytokine secreted by activated T cells and macrophages.

Applications Reported:
MP1-31G6 has been reported for use in detection of mouse GM-CSF by ELISA.

Applications Tested:
This antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse GM-CSF in combination with the purified MP1-22E9 (Cat. No. 14-7331) antibody for capture and recombinant mouse GM-CSF (Cat. No. 14-8331) as the standard.
A suitable range of concentrations for ELISA detection is 0.5–2 µg/mL.
A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL – 8 pg/mL should be included in each ELISA plate.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

The GM-CSF gene encodes a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active protein exists as a homodimer in the extracellular space.
This gene is located in a cluster of related genes at chromosome region 5q31, associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia.
Other genes in the cluster include interleukins 4, 5, and 13.
The gene promotes tissue inflammation, and elevated cytokine levels have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome.
Mice lacking this gene or its receptor develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.