Thermo Fisher Scientific GM-CSF Monoclonal Antibody (MP1-31G6), Biotin, eBioscience

Applications

Tested Dilution

Publications

ELISA (ELISA)

0.5-2 µg/mL

View 1 publication 1 publication

Product Specifications

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MP1-31G6

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466948

Product Specific Information

Description: The MP1-31G6 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF), a 14 kDa cytokine secreted by activated T cells and macrophages.

Applications Reported: MP1-31G6 has been reported for use in detection of mouse GM-CSF by ELISA.

Applications Tested: The MP1-31G6 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) in combination with the purified MP1-22E9 (14-7331) antibody for capture and recombinant mouse GM-CSF (14-8331) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.