Thermo Fisher Scientific IFN gamma Monoclonal Antibody (R4-6A2), Biotin, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 2 publications 2 publications

ELISA (ELISA)

0.5-2 µg/mL

View 52 publications 52 publications

Functional Assay (Functional)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

R4-6A2

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• Functional Grade
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466938

Product Specific Information

Description: The R4-6A2 antibody reacts with mouse interferon-gamma (IFN-gamma ), a homodimeric 15-20 kDa cytokine secreted by Th1 cells. The R4-6A2 antibody is a neutralizing antibody. IFN-gamma is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.

Applications Reported: The R4-6A2 antibody has been reported for use in immunoblotting (WB), ELISPOT, and ELISA.

Applications Tested: The biotinylated R4-6A2 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IFN gamma (Interferon-gamma, IFN-g) in combination with the purified AN-18 (14-7313) antibody or the purified XMG1.2 (14-7311) antibody for capture and recombinant mouse IFN gamma (39-8311) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL.

A standard curve consisting of doubling dilutions of the recombinant standard over the range of 200 pg/mL - 1.6 pg/mL (AN-18 assay) or 2000 pg/mL - 15 pg/mL (XMG1.2 assay) should be included in each ELISA plate.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.