Thermo Fisher Scientific TNF alpha Polyclonal Antibody, Biotin, eBioscience
마우스 및 랫트 TNF alpha를 인식하는 비오틴 표지 폴리클로날 항체입니다. ELISA 및 ELISPOT에 사용 가능하며, 0.5–2 µg/mL 농도 범위에서 최적화되었습니다. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공합니다. 4°C에서 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | - | View 1 publication |
| ELISA (ELISA) | 0.5–2 µg/mL | View 14 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466950 |
Product Specific Information
The affinity-purified polyclonal rabbit antibody reacts with mouse and rat tumor necrosis factor-alpha (TNFα). Mouse TNFα is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4 T cells, and NK cells, with a 26 kDa membrane-bound form. TNFα is cytolytic and plays a key role in immune regulation. Bioactive TNFα exists as a homotrimer.
Applications Reported: ELISA and ELISPOT.
Applications Tested:
This biotinylated rabbit anti-mouse/rat TNFα polyclonal antibody is tested as the detection antibody in a sandwich ELISA for mouse or rat TNFα, used with the monoclonal capture antibody clone TN3.19 (14-7423) and recombinant TNFα (14-8321) as standard.
Recommended detection range: 0.5–2 µg/mL.
Standard curve range:
- Mouse TNFα: 2000–15 pg/mL
- Rat TNFα: 5000–30 pg/mL
Recognition of mouse TNFα is not interfered with by 1000x excess soluble TNFα receptor type I or II.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNFα regulates immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.
It exists as multimers (dimers, trimers, or pentamers) and shows a 17 kDa band under non-reducing SDS-PAGE conditions. Mouse knockout studies indicate neuroprotective roles, and TNFα induces tumor necrosis in tumor-bearing mice. It is involved in immune responses to bacterial, viral, parasitic, and fungal infections and plays a role in tumor necrosis. TNFα acts synergistically with interferon-gamma and has been implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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