Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (SPRCL5), Biotin, eBioscience
Mouse CD185(CXCR5) 인식 SPRCL5 클론의 Biotin 결합 단클론 항체로, Flow Cytometry 및 IHC에 적합. B세포 및 T세포의 CXCR5 발현 분석에 활용. 0.5 mg/mL 농도, 4°C 암소 보관. Affinity chromatography로 정제된 고품질 연구용 시약.
- 카탈로그번호
- 13-7185-8x (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (SPRCL5), Biotin, eBioscience
Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (SPRCL5), Biotin, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 13 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPRCL5 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572799 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugate Formats
- APC
- Brilliant UV 615
- Brilliant UV 805
- FITC
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Request custom conjugation
Product Specific Information
This SPRCL5 monoclonal antibody reacts with Mouse CD185 (CXCR5), also known as Burkitt lymphoma receptor 1 (BLR1). CXCR5 is a seven-transmembrane G protein-coupled receptor originally identified in Burkitt’s lymphoma. It is expressed on B cells, CD4+ T cells (excluding Th1 and Th2 subsets), and a subpopulation of memory (CD45RO+) T cells.
CXCR5+ circulating T cells migrate to lymph nodes via CCR7 and CD62L expression. In tonsil, CXCR5 is expressed on nearly all CD4+ cells along with activation markers CD69 and ICOS. Tonsillar CXCR5+ cells induce antibody production when co-cultured with B cells, supporting their helper function. CXCR5 plays a critical role in lymphocyte trafficking, particularly T cell migration into B cell follicles of germinal centers in response to CXCL13, making it a marker of follicular helper T cells.
Note: SPRCL5 does not recognize formaldehyde-fixed epitopes; staining should be performed prior to fixation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes. Recommended ≤1 µg/test in 100 µL volume, with cell numbers ranging from 10⁵–10⁸ cells/test. Optimize antibody titration for best results.
Target Information
CXCR5 (C-X-C Chemokine Receptor Type 5, CD185), also known as Burkitt lymphoma receptor 1 (BLR1), is a G protein-coupled seven-transmembrane receptor for chemokine BLC. It is expressed in lymphatic tissues such as lymph node follicles and spleen, and plays an essential role in B cell migration.
CXCR5 expression has been reported in human and animal lymphatic organs, blood, bone marrow, brain, liver, lymph node, skin, spleen, stomach, and tonsil. It is part of the large GPCR family, which shares a seven-helical transmembrane structure and mediates diverse signaling pathways via G-proteins and downstream effectors. GPCRs are key pharmacological targets, with drugs such as Zyprexa and Claritin developed for mental health and allergy applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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