Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (SPRCL5), Biotin, eBioscience

Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (SPRCL5), Biotin, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• APC
• Brilliant UV 615
• Brilliant UV 805
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation

Product Specific Information

This SPRCL5 monoclonal antibody reacts with Mouse CD185 (CXCR5), also known as Burkitt lymphoma receptor 1 (BLR1). CXCR5 is a seven-transmembrane G protein-coupled receptor originally identified in Burkitt’s lymphoma. It is expressed on B cells, CD4+ T cells (excluding Th1 and Th2 subsets), and a subpopulation of memory (CD45RO+) T cells.

CXCR5+ circulating T cells migrate to lymph nodes via CCR7 and CD62L expression. In tonsil, CXCR5 is expressed on nearly all CD4+ cells along with activation markers CD69 and ICOS. Tonsillar CXCR5+ cells induce antibody production when co-cultured with B cells, supporting their helper function. CXCR5 plays a critical role in lymphocyte trafficking, particularly T cell migration into B cell follicles of germinal centers in response to CXCL13, making it a marker of follicular helper T cells.

Note: SPRCL5 does not recognize formaldehyde-fixed epitopes; staining should be performed prior to fixation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes. Recommended ≤1 µg/test in 100 µL volume, with cell numbers ranging from 10⁵–10⁸ cells/test. Optimize antibody titration for best results.

Target Information

CXCR5 (C-X-C Chemokine Receptor Type 5, CD185), also known as Burkitt lymphoma receptor 1 (BLR1), is a G protein-coupled seven-transmembrane receptor for chemokine BLC. It is expressed in lymphatic tissues such as lymph node follicles and spleen, and plays an essential role in B cell migration.

CXCR5 expression has been reported in human and animal lymphatic organs, blood, bone marrow, brain, liver, lymph node, skin, spleen, stomach, and tonsil. It is part of the large GPCR family, which shares a seven-helical transmembrane structure and mediates diverse signaling pathways via G-proteins and downstream effectors. GPCRs are key pharmacological targets, with drugs such as Zyprexa and Claritin developed for mental health and allergy applications.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.