Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa Ig superfamily member expressed by macrophages, dendritic cells, activated B cells, and activated T cells. CD80 binds to CD28 and CD152 (CTLA-4) on T cells, playing a crucial role in T-B cell communication and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes, ≤0.25 µg/test
• Recommended Use: Optimize titration per assay
• Fluorochrome: Brilliant Violet™ 711 (BV711), emission 713 nm, for 405 nm laser cytometers

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to reduce non-specific interactions.

Fixation & Storage:
Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark. BV711 is not compatible with methanol-based fixation.

Light Sensitivity:
Protect from light to avoid photo-induced oxidation.

Excitation/Emission:
407 nm / 713 nm (Violet Laser)

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on antigen-presenting cells and essential for T cell activation. CD80 is rapidly induced on activated B cells, EBV-transformed lines, Burkitt’s lymphoma cells, T cells, and monocytes. Dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures.

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