Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.03 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 561
• Alexa Fluor 660
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 650 / 786
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 780
• Request custom conjugation

Product Specific Information

The FN50 monoclonal antibody reacts with human CD69 (Very Early Activation Antigen, VEA), a 30 kDa disulfide-linked dimer expressed on activated lymphocytes, monocytes, and platelets. CD69 is rapidly upregulated upon activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells
  • Use at 5 µL (0.03 µg) per test
  • Defined for 100 µL total staining volume (10⁵–10⁸ cells/test)

Brilliant Violet™ 711 (BV711) emits at 713 nm and is suitable for cytometers with a 405 nm violet laser. Ensure the instrument supports this fluorochrome.

When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant UV™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42) or
• Brilliant Stain Buffer™ (Product # 00-4409-75)
  to minimize non-specific polymer interactions.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)

BV711 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 713 nm
Laser: Violet laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is present on activated platelets. CD69 is involved in lymphocyte signal transduction and proliferation, and may play roles in NK cell and platelet signaling.
Disorders associated with CD69 dysfunction include coccidioidomycosis and asthma.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without authorization.

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