Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.25 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 647
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 786
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 700 / 725 / 755
• NovaFluor Yellow 610 / 690 / 700 / 755
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

Description:
The IT2.2 monoclonal antibody reacts with human CD86 (B7-2), an ~80 kDa surface receptor. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and is upregulated upon stimulation via BCR complex, CD40, and cytokine receptors. CD86 interacts with CD28 and CTLA-4 on T cells, playing a vital role in T-B cell crosstalk, costimulation, and immune regulation.

Applications Reported:
Validated for flow cytometric analysis.

Applications Tested:
Pre-diluted and tested on normal human peripheral blood cells. Recommended use: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Violet™ 711 (BV711) emits at 713 nm and is designed for use with a violet (405 nm) laser. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples may be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark. BV711 is not compatible with methanol-based fixation.

Excitation/Emission:
407 nm / 713 nm (Violet Laser)

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD86 is a ligand for CTLA4 and CD28, acting as a costimulatory molecule in T-cell activation. Insufficient co-stimulation via CD86/CD80 may induce tolerance. CD86 is a type I membrane protein expressed by antigen-presenting cells, binding CD28 for activation or CTLA-4 for inhibition of T-cell responses. Alternative splicing produces multiple isoforms. Dysregulation of CD86 is associated with conditions such as gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.