Invitrogen™

Mouse RiboPure™-Blood RNA Isolation Kit with Manual

For the purification of extremely high-quality total RNA (and optionally, microRNA) from mouse or rat blood in about 35 minutes.자세히 알아보기

For the purification of extremely high-quality total RNA (and optionally, microRNA) from mouse or rat blood in about 35 minutes. The Ambion™ kit includes sufficient reagents for 25 RNA purifications from whole blood samples ranging from 0.1–0.5 each.

• Includes RNA_later_™, predispensed in 2 mL tubes, for RNA stabilization
• Resulting RNA is suitable for stringent downstream applications such as array analysis and qRT-PCR

Due to interference from the enzymatic inhibitors and anticoagulants present in blood samples, RNA purification from whole blood requires a stringent isolation method that yields high-quality RNA depleted of inhibiting agents and contaminants. The kit includes microfuge tubes preloaded with RNA_later_™ Tissue Collection:RNA Stabilization Solution, an aqueous, nontoxic storage reagent that prevents RNA degradation and preserves RNA profiles within samples. Stabilized samples can be stored at room temperature for up to 3 days or at –20°C for long-term storage. The related Figures show the performance of RNA isolated with the Mouse RiboPure™-Blood Kit in real-time RT-PCR. The kit includes an alternative protocol for isolating total RNA that contains microRNA. Typically, 30–55 μg of total RNA (containing microRNA) is obtained from 0.5 mL mouse blood. Yields from rat blood are considerably more variable, generally 18–40 μg from 0.5 mL blood.

사양

형식Filter Cartridge

Isolation TechnologyOrganic Extraction, Spin Column

정제 시간35 min.

종Mouse

샘플 종류Blood

최종 제품 유형Total RNA, micro RNA

고처리량 호환성Not High-throughput Compatible (Manual)

용도(애플리케이션)Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting

포함Acid–Phenol:Chloroform, 3M sodium acetate pH 5.5, wash solution 1 concentrate, wash solution 2/3 concentrate, 2mL tubes pre-loaded with RNA_later_, lysis solution, filter cartridges, collection tubes, nuclease-free water and elution solution

프렙 스케일100 μL to 1 mL Blood Samples (Medium-scale)

제품라인Ambion™, RiboPure™

제품 유형Kit

수량25 preps

배송 조건Room Temperature

시작 물질 양Up to 0.5 ml whole blood

TargetmiRNA, Total RNA

수율40 μg

Unit SizeEach