Thermo Fisher Scientific LeukoLOCK Fractionation & Stabilization Kit, 20 preps

Invitrogen™ LeukoLOCK™ Fractionation & Stabilization Kit with Manual

The Ambion™ LeukoLOCK™ Fractionation & Stabilization Kit is one of two kits that comprise the Total RNA Isolation System (patent pending) — an innovative method for cellular fractionation of whole blood and total RNA stabilization and extraction from the leukocyte population. The kit includes sufficient reagents for 20 reactions and is optimized for use with human blood.

Blood contains rich cellular information; however, globin mRNA in RNA prepared from whole blood can interfere with downstream expression profiling. The LeukoLOCK™ System employs filter-based leukocyte-depletion technology to isolate leukocytes from whole blood and uses Ambion’s RNA_later™ Solution to stabilize the cells on the filter. By excluding red blood cells, the RNA purified from captured leukocytes is inherently depleted of globin mRNA, improving sample quality for expression profiling and other applications.

LeukoLOCK™ Fractionation & Stabilization Kit Overview

A 9–10 mL sample of anticoagulated blood is passed through a LeukoLOCK™ filter, which captures the total leukocyte population while eliminating red blood cells (including reticulocytes), platelets, and plasma. After rinsing with phosphate-buffered saline (PBS), the filter is flushed with RNA_later™ Solution to stabilize the RNA in the captured leukocytes.
The RNA can be isolated immediately, or stabilized cells can be maintained for several days at room temperature, or for longer periods at –20°C or –80°C.

Accessory Products

The LeukoLOCK™ Fractionation & Stabilization Kit is designed for use with the LeukoLOCK™ Total RNA Isolation Kit (Cat. No. AM1934).
These kits can be used separately or together as the LeukoLOCK™ Total RNA Isolation System (Cat. No. AM1923), allowing flexibility when blood collection and RNA processing occur at different locations.

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