Invitrogen™

LeukoLOCK™ Fractionation & Stabilization Kit with Manual

The Ambion™ LeukoLOCK™ Fractionation & Stabilization Kit is one of two kits that comprise the Total RNA Isolation System (patent자세히 알아보기

The Ambion™ LeukoLOCK™ Fractionation & Stabilization Kit is one of two kits that comprise the Total RNA Isolation System (patent pending), an innovative method for cellular fractionation of whole blood and total RNA stabilization and extraction from the leukocyte population. The kit includes sufficient reagents for 20 reactions. The LeukoLOCK™ System (Cat. No. AM1923) is optimized for use with human blood. Blood is a storehouse of cellular information; however, the presence of globin mRNA in RNA prepared from whole blood can interfere with downstream expression profiling applications. The LeukoLOCK™ System employs filter-based leukocyte-depletion technology to isolate leukocytes from whole blood and Ambion`s RNA_later_™ Solution to stabilize the cells on the filter. By excluding red blood cells, the RNA that is purified from captured leukocytes is inherently depleted of globin mRNA, which improves sample utility for expression profiling and other applications (see Figure).

LeukoLOCK™ Fractionation & Stabilization Kit
A 9–10 mL sample of anticoagulated blood is passed through a LeukoLOCK™ filter, which captures the total leukocyte population while eliminating red blood cells (including reticulocytes), platelets, and plasma. After rinsing with phosphate-buffered saline, the filter is flushed with RNA_later__™_ Solutionto stabilize the RNA in the captured leukocytes (see protocol schematic). The RNA can be isolated immediately, or stabilized cells can be maintained for several days at room temperature or for longer periods at –20°C or –80°C (see Figure).

Accessory Products:
LeukoLOCK™ Fractionation & Stabilization Kit is designed for use with the LeukoLOCK™ Total RNA Isolation Kit (Cat. No. AM1934). These kits are offered separately to accommodate blood sample collection at one location and sample processing at a different location, but may be purchased together as the LeukoLOCK™ Total RNA Isolation System (Cat. No. AM1923).

사양

농도1X

Isolation TechnologyVacuum Filter Column, Magnetic Bead

샘플 종류Blood

최종 제품 유형Cells (Leukocytes)

고처리량 호환성Not High-throughput Compatible (Manual)

용도(애플리케이션)Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting

포함RNA_later_ Solution, Rubber Septum Caps, White Slip Connectors, Transfer Spikes, LeukoLOCK Filters, and Elution Solution, and 1X PBS

프렙 스케일> 1 mL Blood Samples (Large-scale)

제품라인Ambion™, LeukoLOCK™

제품 유형Kit

수량20 preps

배송 조건Room Temperature

시작 물질 양Up to 10 ml whole blood

TargetTotal RNA

수율20 μg

Unit SizeEach