Thermo Fisher Scientific TRPC5 Monoclonal Antibody (N67/15), PE

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

Assay-dependent

-

Antibody Microarray (AM)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

N67/15

Immunogen

Synthetic peptide amino acids 827-845 of human TrpC5 (also known as short transient receptor potential channel 5, and Htrp5) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRPC5,uniProtId:Q9UL62-1,ncbiNodeId:9606,antigenRange:827-845,antigenLength:973,antigenImageFileName:MA5-45480_TRPC5_Q9UL62-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45480_TRPC5_Q9UL62-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PE PE PE

View additional formats

• Unconjugated
• APC
• FITC
• PerCP
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931934

Product Specific Information

1 µg/mL of MA5-45480 was sufficient for detection of TrpC5 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 110kDa.

Target Information

TRPC5 belongs to the transient receptor family. TRPC5 is one of the seven mammalian TRPC (transient receptor potential channel) proteins. The TRPC5 protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC5 is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. TRPC5 also interacts with multiple proteins including calmodulin, CABP1, enkurin, Na(+)-H+ exchange regulatory factor (NHERF ), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1). TRPC5 is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC5 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, or G-protein coupled receptors. TRPC5 has also been shown to be calcium-selective, and may also be activated by intracellular calcium store depletion. Diseases associated with TRPC5 include Hypertrophic Pyloric Stenosis and Pyloric Stenosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.