Thermo Fisher Scientific NOTCH1 Monoclonal Antibody (mN1A)
NOTCH1 단백질을 인식하는 Mouse monoclonal antibody (clone mN1A). Western blot, IHC, Flow cytometry, Immunoprecipitation에 적합. Human 및 Mouse 시료 반응. Protein G 정제, PBS buffer, 1 mg/mL 농도, 장기보관 시 -20°C 권장.
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Thermo Fisher Scientific NOTCH1 Monoclonal Antibody (mN1A)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:1,000 | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 10 µL / 1×10^6 cells | - |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | mN1A |
| Immunogen | Synthetic peptide corresponding to cdc10-NCR region within mouse Notch 1 |
| Conjugate | Unconjugated |
| Available Formats | FITC Conjugate, Custom Conjugation Request |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_934908 |
Product Specific Information
Membrane permeabilization is required for flow cytometry applications.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10^6 cells in 100 µL.
Mouse anti-Mouse Notch 1 antibody, clone mN1A, recognizes Notch 1—one of four major transmembrane receptors (Notch 1–4) in the Notch signaling pathway. This pathway is activated through binding to DSL domain-containing membrane-bound ligands.
Target Information
The Notch gene family encodes transmembrane proteins with multiple cysteine-rich EGF-like repeats in the extracellular domain.
Four Notch genes (Notch1–4) are described in mammals and are involved in differentiation of the nervous system and other tissues.
EGF-like proteins Delta and Serrate act as ligands for Notch1.
Mature Notch proteins form heterodimeric receptors composed of an extracellular subunit (NEC) and a transmembrane subunit (Ntm), generated by cleavage of Notch pre-proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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