Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (AD1.1.10), FITC

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA1-81891 recognizes proteins containing the motif H-H-H-H-H-H, making it valuable for detecting His-tagged proteins.
It can be used to track purification of proteins expressed in bacteria, yeast, or insect cells and reacts with all His-tagged proteins tested so far.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at either the N- or C-terminal of recombinant proteins to enable isolation or purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of proteins lacking specific antibodies by fusing a known epitope.
His-tagged proteins are purified via metal chelate chromatography due to strong Ni²⁺ affinity of histidine clusters.
His tag-specific antibodies facilitate detection and co-immunoprecipitation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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