Thermo Fisher Scientific DYKDDDDK Monoclonal Antibody (29E4.G7), DyLight 680
DYKDDDDK(FALG) 태그 인식용 DyLight 680 결합 단클론 항체로, 단백질 발현 및 정량 분석에 적합. ICC/IF, Flow Cytometry, ELISA 등 다양한 응용 가능. 높은 특이성과 안정성을 제공하며, 장기 보관 시 -20°C에서 안정적 유지.
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Thermo Fisher Scientific DYKDDDDK Monoclonal Antibody (29E4.G7), DyLight 680
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | 1:10,000–1:25,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host/Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 29E4.G7 |
| Immunogen | Produced by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag (DYKDDDDK) conjugated to KLH using maleimide. Glycine and cysteine residues added to termini for coupling. |
| Conjugate | DyLight™ 680 |
| Excitation/Emission Max | 676/705 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Formats
- Unconjugated
- Agarose
- Biotin
- DyLight 405
- DyLight 488
- DyLight 549
- DyLight 649
- DyLight 800
- FITC
- HRP
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge if not clear after standing at room temperature. Stable for several weeks at 4°C as undiluted liquid; dilute only prior to immediate use.
This antibody is directed against the FLAG™ epitope tag and is useful for detecting overexpressed proteins in various assays. It recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of target proteins in transfected or transformed cells.
Target Information
The DYKDDDDK tag (FLAG tag) is a short peptide sequence used for protein purification, detection, and localization. It consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. Commonly fused to the N- or C-terminus of proteins, it enables easy identification and isolation using anti-FLAG antibodies. Widely used in molecular biology and biochemistry, it supports protein-protein interaction studies, expression analysis, and localization research. The FLAG tag is hydrophilic, minimizing interference with protein function.
FLAG™ is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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