Thermo Fisher Scientific 6X His Tag Monoclonal Antibody (33D10.D2.G8), DyLight 680
6X His 태그 단백질 검출용 DyLight 680 결합 단클론 항체. Western blot, ICC/IF, ELISA에 적합. 높은 특이성과 재현성 제공. 단백질 정제 및 발현 검증 연구용. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | >1:10,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 33D10.D2.G8 |
| Immunogen | Synthetic peptide corresponding to the 6X HIS epitope tag (H-H-H-H-H-H) conjugated to KLH using maleimide |
| Conjugate | DyLight™ 680 |
| Excitation / Emission Max | 676 / 705 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats Available
- Unconjugated
- Atto 550
- Atto 647N
- Biotin
- Cyanine3
- Cyanine5
- DyLight 405
- DyLight 488
- DyLight 549
- DyLight 649
- DyLight 800
- HRP
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge if not clear after standing at room temperature. Stable for several weeks at 4°C as undiluted liquid. Dilute only prior to immediate use.
This monoclonal antibody is directed against the 6X His motif and is useful for detecting over-expressed proteins containing the 6X His epitope tag. It reacts with all His-tagged proteins tested and shows no cross-reactivity with endogenous bacterial proteins in Western blotting. Recognizes His-tag fused to either N- or C-termini of target proteins in transfected or transformed cells.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). Commonly expressed at N- or C-terminal regions of recombinant proteins, it enables purification by immobilized metal affinity chromatography (IMAC). Epitope tagging allows detection of recombinant proteins even when specific antibodies are unavailable. His-tagged proteins bind selectively to metal ions such as Ni²⁺, enabling efficient purification. His tag-specific antibodies facilitate detection and co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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