Thermo Fisher Scientific Phospho-AKT (Thr308) Monoclonal Antibody (18F3.H11), DyLight 649
Phospho-AKT (Thr308) 단백질을 검출하는 DyLight 649 형광 표지 단일클론 항체로, 인간 및 생쥐 시료에 반응합니다. Western blot, ICC/IF, Flow cytometry, ELISA 등 다양한 응용에 적합하며, 고순도 Protein A 정제 및 안정한 형광 특성 제공.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | >1:10,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | 1:4,000–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 18F3.H11 |
| Immunogen | Synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein |
| Conjugate | DyLight™ 649 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- Biotin
- DyLight 488
- DyLight 549
- Request custom conjugation
Product Specific Information
- Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This antibody is specific for human and mouse AKT protein phosphorylated at T308.
BLAST analysis suggests cross-reactivity with AKT pT308 from most vertebrate species based on 100% homology with the immunizing sequence.
Cross-reactivity with AKT2 and AKT3 will likely occur.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase regulating cell survival. The activated enzyme inhibits apoptosis and promotes cell cycle progression by phosphorylating multiple targets in various cell types, including cancer cells.
Akt is activated by insulin, PI3K, IGF1, and other growth factors, promoting survival via inhibition of apoptosis through phosphorylation and inactivation of forkhead transcription factors and caspase-9.
The AKT pathway is a major focus in cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures.
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