Thermo Fisher Scientific MEK1 C-Term Monoclonal Antibody (13B6.G12), DyLight 680
MEK1 단백질의 C-말단을 인식하는 Mouse monoclonal antibody. DyLight 680 형광 표지로 Western blot과 ELISA에 적합. Human, Mouse, Rat 반응성. 고순도 Protein A 정제, 안정적 형광 신호 제공.
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Thermo Fisher Scientific MEK1 C-Term Monoclonal Antibody (13B6.G12), DyLight 680
Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 13B6.G12 |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH |
| Conjugate | DyLight™ 680 |
| Excitation / Emission Max | 676 / 705 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- Biotin
- DyLight 800
- FITC
- HRP
- Custom conjugation available upon request
Product Specific Information
- Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid freeze/thaw cycles. Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid. Dilute only prior to immediate use.
- Reacts specifically with human MEK1.
- Purified from tissue culture supernatant by Protein A.
- Cross-reactivity expected with human, mouse, and rat.
- Does not react with MEK2 isoform.
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase involved in MAPK cascades, regulating cell growth and differentiation. It is activated by phosphorylation of serine residues 218 and 222 by Raf1. MEK1 is implicated in stress response, apoptosis, and cytokine-induced proliferation. Mutations in MAP2K1 can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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