Thermo Fisher Scientific GFP Monoclonal Antibody (9F9.F9), DyLight 680

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Unconjugated
• AP
• Biotin
• DyLight 405
• DyLight 488
• DyLight 549
• DyLight 649
• DyLight 800
• FITC
• HRP
• Rhodamine
• Custom conjugation available upon request

Product Specific Information

Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (Aequorea victoria) by Western blot. No reaction is seen against RFP.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP is widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular systems.

As a fusion tag, GFP enables localization studies of proteins, tracking their movement, and analyzing subcellular dynamics. GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autocatalytically. The wild-type protein absorbs blue light (395 nm) and emits green light (508 nm) without the need for additional cofactors. GFP fluorescence is stable, species-independent, and suitable for various applications including gene expression monitoring, protein localization, protein-protein interaction (yeast two-hybrid), and FRET-based distance measurements.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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