Thermo Fisher Scientific GFP Monoclonal Antibody (9F9.F9), DyLight 680
GFP 단백질을 인식하는 마우스 단클론 항체로 DyLight 680 형광 표지. Western blot, ICC/IF, ELISA에 적합. 높은 특이성과 신호 안정성을 제공하며, 다양한 형광 분석에 활용 가능. 연구용으로 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9F9.F9 |
| Immunogen | BALB/c mice were immunized with a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. |
| Conjugate | DyLight™ 680 |
| Excitation / Emission Max | 676 / 705 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Available Formats
- Unconjugated
- AP
- Biotin
- DyLight 405
- DyLight 488
- DyLight 549
- DyLight 649
- DyLight 800
- FITC
- HRP
- Rhodamine
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (Aequorea victoria) by Western blot. No reaction is seen against RFP.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP is widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular systems.
As a fusion tag, GFP enables localization studies of proteins, tracking their movement, and analyzing subcellular dynamics. GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autocatalytically. The wild-type protein absorbs blue light (395 nm) and emits green light (508 nm) without the need for additional cofactors. GFP fluorescence is stable, species-independent, and suitable for various applications including gene expression monitoring, protein localization, protein-protein interaction (yeast two-hybrid), and FRET-based distance measurements.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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