Thermo Fisher Scientific CD40 Monoclonal Antibody (HM40-3), Functional Grade, eBioscience
Mouse 및 Rat CD40 인식 단클론 항체로, Flow Cytometry 및 Functional Assay에 적합. B 세포 활성화 및 CD154 결합 억제 연구에 활용 가능. 1 mg/mL 농도의 액상 형태로 제공되며, 4°C에서 보관. 높은 순도(>90%)와 낮은 엔도톡신 수준(<0.001 ng/µg)을 보장.
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Thermo Fisher Scientific CD40 Monoclonal Antibody (HM40-3), Functional Grade
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | 24 publications |
| Functional Assay | Assay-Dependent | 6 publications |
| Inhibition Assay (IA) | - | 1 publication |
| In vitro Assay (IV) | - | 2 publications |
| Miscellaneous PubMed | - | 3 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HM40-3 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865717 |
Available Formats
Product Specific Information
Description:
The HM40-3 monoclonal antibody reacts with mouse and rat CD40, a 45–50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells.
CD40 regulates B cell development and maturation by inducing Ig isotype switching. In combination with IL-4, it protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39) on T cells is crucial for T-B cell crosstalk and immune regulation.
Applications Reported:
- Flow cytometric analysis
- Inhibition of soluble CD154 binding to CD40
- In vitro activation of B cells
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤1 µg per test (100 µL final volume). Cell count may vary (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.
Storage and Handling:
Use in a sterile environment.
Additional Quality Information:
- Filtration: 0.2 µm post-manufacturing filtered
- Purity: >90% (SDS-PAGE)
- Endotoxin Level: <0.001 ng/µg antibody (LAL assay)
- Aggregation: <10% (HPLC)
Target Information
CD40 is a single-chain glycoprotein belonging to the TNFR/NGF superfamily and homologous to CD30. It is expressed on all B cells except plasma cells, and also on epithelial cells, carcinomas, and lymphoid dendritic cells.
CD40 plays a key role in immune and inflammatory responses, including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
AKNA transcription factor regulates CD40 expression, enabling homotypic cell interactions. TNFR2 acts as a signal transduction mediator with CD40.
CD40-CD154 interaction is implicated in amyloid-beta-induced microglial activation, an early event in Alzheimer’s disease.
Two alternatively spliced transcript variants encoding distinct isoforms have been reported.
Diseases associated with CD40 dysfunction include Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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