Thermo Fisher Scientific CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), Functional Grade, eBioscience

Thermo Fisher Scientific CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 555
• NovaFluor Blue 660-120S
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation available

Product Specific Information

The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alpha IIb). CD41 is expressed on platelets, megakaryocytes, and hematopoietic progenitors. It associates with CD61 (gpIIIa, integrin beta III) to form a receptor crucial for platelet adhesion and aggregation via fibrinogen, fibronectin, and vitronectin binding.

CD41 expression is useful for identifying hematopoietic stem cells (HSCs) when combined with SLAM-family markers CD48 and CD150. CD150⁺CD48⁻CD41⁻ cells are highly efficient in long-term multilineage reconstitution.

• Applications Reported: Flow cytometry and functional assays
• Applications Tested: Flow cytometric analysis of mouse platelets at ≤0.25 µg/test (100 µL volume, 10⁵–10⁸ cells/test; titrate for optimal performance)

Storage and Handling: Use in sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

CD41 (platelet glycoprotein IIb, ITGA2B) consists of alpha (120 kDa) and beta (23 kDa) subunits that interact with CD61 in the presence of calcium to form a functional adhesive receptor. It mediates platelet aggregation and binds to von Willebrand factor, fibrinogen, fibronectin, and vitronectin. CD41 is mainly expressed in the megakaryocyte-platelet lineage and during early hematopoietic differentiation.
Associated diseases include Glanzmann Thrombasthenia and Platelet type-16 bleeding disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.