Thermo Fisher Scientific Sarcomeric Actinin Alpha 2/ACTN2 Monoclonal Antibody (ACTN2, 3293)
ACTN2 단백질을 인식하는 mouse monoclonal 항체로, Western blot, IHC 등 다양한 응용에 사용 가능. Human 시료 반응성. Protein A/G 정제, PBS buffer 내 0.05% BSA 및 sodium azide 포함. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ACTN2, 3293 |
| Immunogen | Recombinant fragment (aa 557–692) of human ACTN2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA-fixed): Incubate antibody for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Alpha actinins belong to the spectrin gene superfamily, representing a group of cytoskeletal proteins including alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types.
- In nonmuscle cells: localized along microfilament bundles and adherens-type junctions, binding actin to the membrane.
- In muscle cells: localized to the Z-disc and dense bodies, anchoring myofibrillar actin filaments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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