Thermo Fisher Scientific Sarcomeric Actinin Alpha 2/ACTN2 Monoclonal Antibody (ACTN2, 3294)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Alpha actinins belong to the spectrin gene superfamily, a group of cytoskeletal proteins including alpha and beta spectrins and dystrophins.
Alpha actinin is an actin-binding protein with multiple roles in different cell types:

• In nonmuscle cells, it is found along microfilament bundles and adherens-type junctions, where it binds actin to the membrane.
• In skeletal, cardiac, and smooth muscle, it localizes to the Z-disc and dense bodies, anchoring myofibrillar actin filaments.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.