Thermo Fisher Scientific Sarcomeric Actinin Alpha 2/ACTN2 Monoclonal Antibody (ACTN2, 3292)
Sarcomeric Actinin Alpha 2 (ACTN2) 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody입니다. Western blot, IHC, Peptide array 등에 사용 가능하며, 고순도 Protein A/G 정제 및 안정적 PBS buffer 포뮬레이션으로 연구용에 적합합니다.
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- 88-MSM2-P0
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- pk
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Thermo Fisher Scientific Sarcomeric Actinin Alpha 2/ACTN2 Monoclonal Antibody (ACTN2, 3292)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ACTN2, 3292 |
| Immunogen | Recombinant fragment (aa557–692) of human ACTN2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Alpha actinins belong to the spectrin gene superfamily, a diverse group of cytoskeletal proteins including alpha and beta spectrins and dystrophins.
Alpha actinin is an actin-binding protein with multiple roles in different cell types.
- In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, binding actin to the membrane.
- In skeletal, cardiac, and smooth muscle cells, isoforms are localized to the Z-disc and analogous dense bodies, anchoring myofibrillar actin filaments.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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