![Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11[APG]), Biotin](https://images.cacheby.com/product-images/cacheby/57056430-28b8-11f0-bf4f-b7c32b12ae43/primary-antibody-image.jpg-650.jpg)
Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11[APG]), Biotin
CD34 항원을 인식하는 Biotin 결합 단일클론 항체로, 인간 유래 세포에서 반응. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. 1 mg/mL 농도의 액상 형태이며, PBS(pH 7.4)에 15mM sodium azide 포함. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4H11[APG] |
| Immunogen | Permanent human cell line from a patient with myeloid leukaemia |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1072193 |
Additional Formats
Product Specific Information
This antibody reacts with extracellular class III epitope on CD34, a 110–115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitor cells and pluripotential stem cells. Expression is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of class III antibodies BIRMA K3 and 8G12 on the KG1a cell line.
Target Information
CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) present on many stem cell populations. It serves as a stem cell marker, with reversible expression on human hematopoietic stem cells. CD34 may act as a receptor involved in endocytosis, adhesion, differentiation, and proliferation of hematopoietic stem and progenitor cells. It may mediate attachment to the bone marrow extracellular matrix or stromal cells and serve as a scaffold for lineage-specific glycans. The intracellular domain can be phosphorylated by protein kinase C, suggesting a role in signal transduction. CD34 dysfunction is associated with dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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