Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (3D5), FITC
FITC 형광 표지된 6x-His Tag 단클론 항체로, 재조합 단백질의 His 태그 검출에 최적화됨. Western blot, ICC/IF, IHC, Flow cytometry 등 다양한 응용 가능. 고순도 정제된 Mouse IgG2b 항체로 498/517 nm 형광 특성. 연구용으로만 사용.
- 카탈로그번호
- R93325
- 판매단위
- pk
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Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (3D5), FITC
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 2 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | View publications |
| Flow Cytometry (Flow) | - | View 2 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D5 |
| Immunogen | 6x His synthetic peptide: C-term -His-His-His-His-His-His-COOH |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.01% sodium azide |
| Storage Conditions | 4°C, store in dark |
| RRID | AB_2556556 |
Available Formats
- Unconjugated
- AP
- HRP
- Custom conjugation available upon request
Product Specific Information
R933-25 was previously sold under product #46-0309. When re-ordering, please use #R933-25.
This antibody detects 6x His tag (C-term) -His-His-His-His-His-His-COOH.
Tested in IF experiments using cultured CHO (Chinese Hamster ovary) cells expressing recombinant epitope-tagged fusion proteins.
For IF, dilute the FITC-conjugated antibody in PBS containing 10% fetal bovine serum (FBS).
Provided amount is sufficient for 25 immunostaining reactions using 1 mL working solution per reaction.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
Commonly expressed at N- or C-terminal regions of recombinant proteins for purification via immobilized metal affinity chromatography (IMAC).
His tag-specific antibodies facilitate detection or co-immunoprecipitation of tagged proteins.
His-tagged proteins can be purified by Ni²⁺-NTA affinity chromatography due to strong and selective chelation of histidine residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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