Thermo Fisher Scientific SIRP alpha Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.5–1 µg/mL

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1 µg/mL

Product Specifications

Product Specific Information

In Western blot applications, this antibody detects a band at approximately 75–110 kDa.
A suggested positive control is THP-1 cell lysate.

Target Information

SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells, and hematopoietic stem cells.
It acts as a substrate for several activated tyrosine kinases such as EGFR, PDGFR, src, and insulin receptor, and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways.

The ligand binding of SIRP alpha to integrin-associated protein CD47 results in phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, mediating recruitment and activation of tyrosine phosphatases SHP-1 and SHP-2.
This interaction regulates processes such as inhibition of macrophage phagocytosis and activation of T cells and DCs.

SIRP alpha influences cellular responses induced by serum, growth factors, insulin, oncogenes, hormones, and cell adhesion, playing a role in various physiological and pathological processes.
Cancer cells often overexpress CD47, activating SIRP alpha and inhibiting macrophage-mediated destruction of cancer cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.