Thermo Fisher Scientific HCN2 Monoclonal Antibody (S71), FITC
HCN2 단백질을 인식하는 FITC 결합 단일클론 항체로, WB, IHC, ICC, IP 등 다양한 응용에 적합합니다. 인간, 생쥐, 랫트 시료에 반응하며, 단백질 G 정제 및 보존제 무첨가 상태로 제공됩니다. 형광 분석용으로 4°C 암소 보관이 권장됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S71 |
| Immunogen | Fusion protein corresponding to amino acids 761–863 (cytoplasmic C-terminus) of rat HCN2 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in the dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931858 |
Available Formats
- Unconjugated (MA5-27718)
- APC (MA5-45403)
- PE (MA5-45406)
- PerCP (MA5-45405)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45404 was sufficient for detection of HCN2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 95 kDa. No cross-reactivity against HCN1.
Target Information
Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain.
HCN2 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides.
HCN1 and HCN2 play important roles in motor learning and neuronal integration by cerebellar Purkinje cells, as well as shaping autonomous neuronal activity and network oscillations.
HCN1 expression is enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus, while HCN2 is abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body.
These regional differences in HCN channel expression contribute to variations in neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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