Thermo Fisher Scientific HCN1 Monoclonal Antibody (S70), PerCP
HCN1 단백질을 인식하는 S70 클론의 마우스 단일클론 항체로, PerCP 형광 표지형입니다. 인간, 마우스, 랫트 반응성이 있으며 WB, IHC, ICC 등 다양한 응용에 적합합니다. 고순도 Protein G 정제, 1 mg/mL 농도, 4°C 보관.
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Thermo Fisher Scientific HCN1 Monoclonal Antibody (S70), PerCP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S70 |
| Immunogen | Fusion protein amino acids 778–910 (C terminus) of rat HCN1 |
| Conjugate | PerCP |
| Excitation/Emission Max | 482/675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931855 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45401 was sufficient for detection of HCN1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 100 kDa. No cross-reactivity against HCN2.
Target Information
HCN1 (Hyperpolarization-activated cation channel 1) is part of the HCN gene family, contributing to spontaneous rhythmic activity in heart and brain. It is activated by hyperpolarization and regulated by cyclic nucleotides.
HCN1 and HCN2 are essential for motor learning and neuronal integration by cerebellar Purkinje cells, influencing autonomous neuronal activity and network oscillations.
HCN1 expression is enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus, while HCN2 is abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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