Thermo Fisher Scientific NFkB p50 Monoclonal Antibody (5D10D11)
Human NFkB p50 단백질을 인식하는 mouse monoclonal antibody로, WB, IHC, ICC, Flow Cytometry, ChIP 등 다양한 응용에 사용 가능. Protein A로 정제된 액상 제품이며, 1 mg/mL 농도. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| ChIP assay (ChIP) | 2 µg/1x10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D10D11 |
| Immunogen | Purified recombinant fragment of human NFKB1 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153885 |
Product Specific Information
MA5-15870 targets NFKB1 in FACS, IF, IHC, ChIP, and WB applications and shows reactivity with Human samples.
Target Information
NFkB is a nuclear transcription factor activated by various extra- and intracellular stimuli such as cytokines, UV radiation, stress, injury, and microbial products. It regulates cellular processes including growth, differentiation, proliferation, apoptosis, and inflammation.
NFKB1 (p50) is a 50 kDa functional subunit of NF-kB and a member of the Rel protein family. It is synthesized as a p105 precursor containing an N-terminal RHD region with nuclear localization signal, DNA-binding, and dimerization domains. NFKB1 (p50) forms homodimers or heterodimers with p65, constituting the functional NFkB complex. This complex is primarily cytoplasmic in an inactive state bound to I-kappa-B. Upon phosphorylation of I-kappa-B, NFkB translocates to the nucleus to modulate gene expression. Phosphorylation of p50 at Ser-337 enhances DNA-binding efficiency. NFkB has been implicated in diseases such as AIDS, hematogenic cancer metastasis, and rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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