Thermo Fisher Scientific Ku80 Monoclonal Antibody (5C5)
Human 및 Mouse 시료에서 XRCC5(Ku80) 단백질을 검출하는 Monoclonal Antibody. Western blot, IHC, ICC, Flow Cytometry, ELISA 등 다양한 응용에 사용 가능. 비결합형(mouse IgG1) 항체로, 4°C 단기 보관 및 -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | View 1 publication |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5C5 |
| Immunogen | Purified recombinant fragment of human XRCC5 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11156182 |
Product Specific Information
MA5-15873 targets XRCC5 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with human and mouse samples.
The immunogen is a purified recombinant fragment of human XRCC5 expressed in E. coli.
MA5-15873 detects XRCC5, which has a predicted molecular weight of approximately 86 kDa.
Target Information
XRCC5 encodes the 80-kDa subunit of the Ku heterodimer protein, also known as ATP-dependent DNA helicase II or DNA repair protein XRCC5.
Ku functions as the DNA-binding component of the DNA-dependent protein kinase and acts with the DNA ligase IV–XRCC4 complex in the repair of DNA double-strand breaks by non-homologous end joining and in V(D)J recombination events.
This gene complements the Chinese hamster xrs-6 mutant defective in DNA double-strand break repair and V(D)J recombination.
A rare microsatellite polymorphism in XRCC5 is associated with cancer susceptibility in patients with varying radiosensitivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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