Thermo Fisher Scientific BLNK Monoclonal Antibody (5G9)
BLNK 단백질을 검출하기 위한 Mouse monoclonal antibody(클론 5G9). Western blot, IHC, ICC/IF, Flow cytometry, ELISA에 사용 가능. Human 및 Mouse 시료에 반응하며, 비결합형 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5G9 |
| Immunogen | Purified recombinant fragment of human BLNK expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11154442 |
Product Specific Information
MA5-15868 targets BLNK in indirect ELISA, FACS, IF, IHC, and WB applications, and shows reactivity with human and mouse samples.
The immunogen is a purified recombinant fragment of human BLNK expressed in E. coli.
MA5-15868 detects BLNK with a predicted molecular weight of approximately 68 kDa.
Target Information
B cell linker protein (BLNK) is encoded by the Blnk gene on chromosome 10 in humans. Also known as SLP-65 (Src homology 2 domain-containing leukocyte protein of 65 kDa), BLNK functions as an adaptor protein in the B cell receptor signaling cascade.
Deficiency in BLNK leads to improper B cell development. BLNK undergoes tyrosine phosphorylation by Syk kinase, providing a platform for interaction with signaling intermediaries such as Vav GEF and Grb2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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