Thermo Fisher Scientific MECP2 Monoclonal Antibody (8H4A5B9)
MECP2 단백질을 인식하는 mouse monoclonal antibody로, Western blot, IHC, ICC, Flow cytometry, ELISA에 적합합니다. 인간 MECP2에 반응하며, Protein G로 정제된 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific MECP2 Monoclonal Antibody (8H4A5B9)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8H4A5B9 |
| Immunogen | Purified recombinant fragment of human MECP2 (aa 7–148) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898482 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3, and MBD4) that contain a methyl-CpG binding domain. MECP2 binds specifically to methylated DNA, a major modification of eukaryotic genomes that plays a crucial role in mammalian development. It can repress transcription from methylated gene promoters and, unlike other MBD family members, is X-linked and subject to X inactivation. MECP2 is essential for embryonic development, and mutations in the MECP2 gene are responsible for most cases of Rett syndrome, a progressive neurological developmental disorder and one of the most common causes of mental retardation in females.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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