Thermo Fisher Scientific AQP2 Polyclonal Antibody
Rabbit polyclonal antibody recognizing human and non-human primate AQP2. Validated for WB and IHC at 1:2,000 dilution. Lyophilized form without preservative, reconstitute in sterile water. Useful for studying vasopressin-regulated water channels in kid...
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | – |
| Immunohistochemistry (IHC) | 1:2,000 | – |
| Miscellaneous (PubMed) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human AQP2 (aa 90–140) conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The peptide is homologous in rat and mouse.
- Specificity: AQP2.
Target Information
Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans. Vasopressin-induced cAMP increase leads to phosphorylation of AQP2 at serine-256 and its translocation from intracellular vesicles to the apical membrane of principal cells. Serine-261 has also been identified as a phosphorylation site that decreases with vasopressin treatment, suggesting its role in vasopressin-dependent AQP2 trafficking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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