Thermo Fisher Scientific 53BP1 Monoclonal Antibody (6B3E10)
53BP1 단백질을 인식하는 Mouse 모노클로날 항체로, Western blot, IHC, Flow cytometry, ELISA 등에 사용 가능. Human 시료 반응성. Protein G 정제, 1 mg/mL 농도, PBS 완충액에 0.05% sodium azide 포함. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6B3E10 |
| Immunogen | Purified recombinant fragment of human TP53BP1 (aa 574–773) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4 °C (short term). For long-term storage, store at −20 °C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898561 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically double-strand breaks. It may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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