Thermo Fisher Scientific Phospho-ERK1/ERK2 (Tyr204) Polyclonal Antibody
ERK1/ERK2의 Tyr204 인산화 상태를 검출하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 적합합니다. 인간 p44/42 MAP Kinase 유래 펩타이드 항원 사용. 고농도(1 mg/mL) 액상 형태로 −20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y(p)-V-A) derived from Human p44/42 MAP Kinase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554433 |
Product Specific Information
- Suggested positive control for Western blot: SK-BR-3, U0126, or LY294002 cells
- Suggested positive control for IHC: Human breast carcinoma
- Suggested positive control for ICC/IF: HeLa cells
Target Information
ERK1 and ERK2 are widely expressed and play key roles in regulating meiosis, mitosis, and postmitotic functions in differentiated cells.
They are activated by various stimuli including growth factors, cytokines, viral infection, and G protein-coupled receptor ligands.
Upon activation, Ras interacts with Raf, which phosphorylates MEK1/2, leading to phosphorylation of ERK1/2.
Activated ERKs phosphorylate cytoplasmic targets or translocate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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