Thermo Fisher Scientific MCP-4 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL
• Publications: 1 publication

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MCP-4 (CCL13). Anti-Human MCP-4 (CCL13)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human MCP-4 by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MCP-4 (CCL13) (500-P04) as a capture antibody, allows detection of at least 2000–4000 pg/mL of Recombinant Human MCP-4.

Western Blot:
To detect Human MCP-4 by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. When used with compatible development reagents, the detection limit for Recombinant Human MCP-4 is 1.5–3.0 ng/lane, under reducing or non-reducing conditions.

Packaging:
500-P04BT-1MG is provided as 2 × 500 µg.

Target Information

Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFα. MCP-4 is a ligand for three different G protein–coupled receptors: CCR2, CCR3, and CCR5. MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils, and basophils, which is associated with allergic responses.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.