Thermo Fisher Scientific PPBP Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific PPBP Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

• AA Sequence of recombinant protein:
  AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KDGRKICLDP DAPRIKKIVQ KKLAGDESAD

• Preparation:
  Produced from sera of goats immunized with highly pure Recombinant Human NAP-2 (CXCL7). Anti-Human NAP-2 (CXCL7)-specific antibody was purified by affinity chromatography and then biotinylated.

• Sandwich ELISA:
  To detect Human NAP-2 by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NAP-2 (CXCL7) (500-P03) as a capture antibody, allows detection of at least 2000–4000 pg/mL of Recombinant Human NAP-2.

• Western Blot:
  To detect Human NAP-2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible development reagents, the detection limit for Recombinant Human NAP-2 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

• Packaging:
  500-P03GBT-1MG will be provided as 2 × 500 µg.

Target Information

Members of the α-chemokine subfamily of inducible, secreted, pro-inflammatory cytokines contain a Cys-X-Cys motif and are chemotactic for neutrophils. Platelet basic protein (PBP), a member of this family, resides in platelet α-granules and is released upon activation. Proteolytic cleavage of PBP generates peptides such as mature PBP, connective tissue-activating peptide III (CTAP III), β-thromboglobulin (β-TG), and neutrophil-activating peptide 2 (NAP-2). These molecules mediate inflammation and wound healing. NAP-2 specifically activates chemotaxis and degranulation in neutrophils during inflammation. The human PBP gene is located at chromosome 4q12–q13.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.